RESUMO
Three sulphur-crested cockatoos (Cacatua galerita) were diagnosed as psittacine beak and feather disease (PBFD). Histopathology of the feather pulp and follicles showed intracytoplasmic botryoid clusters or granular inclusion bodies in epithelial cells and macrophages. Electron microscopy revealed multiple cytoplasmic clusters of electron dense viral particles corresponding to the inclusions. PBFD virus (circovirus) DNA-specific product was detected from formalin-fixed paraffin-embedded feathers by nested polymerase chain reaction (PCR) method.
Assuntos
Doenças das Aves/virologia , Infecções por Circoviridae/veterinária , Circovirus/crescimento & desenvolvimento , Psittaciformes , Animais , Infecções por Circoviridae/patologia , Infecções por Circoviridae/virologia , Circovirus/genética , Circovirus/ultraestrutura , DNA Viral/genética , Plumas/ultraestrutura , Plumas/virologia , Corpos de Inclusão Viral/ultraestrutura , Microscopia Eletrônica/veterinária , Reação em Cadeia da Polimerase/veterinária , TailândiaRESUMO
A retrospective study for the detection of porcine circovirus 2 (PCV-2) DNA was conducted by nested PCR method in 16 cases of swine post-weaning multisystemic wasting syndrome (PMWS) in Thailand. Histopathology showed characteristic lesions of PMWS and intracytoplasmic viral inclusion bodies in macrophages infiltrating in lymphoid tissues. PCV-2 DNA was detected from formalin-fixed and/or formalin-fixed paraffin-embedded tissues from all pigs with PMWS. The amplified products were digested with Hae III.
